La Vitamina B-12, inhibe los retrovirus

[Merce]

B12 inhibits Retroviruses

Inhibition of productive human immunodeficiency virus-1 infection by cobalamins

Various cobalamins act as important enzyme cofactors and modulate cellular function. We investigated cobalamins for their abilities to modify productive human immunodeficiency virus-1 (HIV-1) infection of hematopoietic cells in vitro. We show that hydroxocobalamin (OH-Cbl), methylcobalamin (Me-Cbl), and adenosylcobalamin Ado-Cbl (Ado-Cbl) inhibit HIV-1 infection of normal human blood monocytes and lymphocytes. The inhibitory effects were noted when analyzing the monocytotropic strains HIV-1-BaL and HIV-1-ADA as well as the lymphocytotropic strain HIV-1-LAI. Cobalamins did not modify binding of gp120 to CD4 or block early steps in viral life cycle, inhibit reverse transcriptase, inhibit induction of HIV-1 expression from cells with established or latent infection, or modify monocyte interferon-alpha production. Because of the ability to achieve high blood and tissue levels of cobalamins in vivo and the general lack of toxicity, cobalamins should be considered as potentially useful agents for the treatment of HIV-1 infection.

Cobalamin Inhibition of HIV-1 Integrase and Integration of HIV-1 DNA into Cellular DNA

Our prior studies showed that certain cobalamins inhibit productive HIV-1 infection of primary cultures of blood lymphocytes and monocytes. We demonstrate here that this antiviral activity may be mediated by an inhibition of HIV-1 integrase, an enzyme required for productive infection. Purified recombinant HIV-1 integrase activity was inhibitedin vitroby hydroxocobalamin (OH-Cbl), methylcobalamin (Me-Cbl), adenosylcobalamin (Ado-Cbl), and dicyanocobinamide (CN2-Cbi) with IC50values of approximately 17, 17, 17, and 4 μM, respectively. The agents inhibited HIV-1 infection of cultured monocytes (IC50values for OH-Cbl, Me-Cbl, Ado-Cbl, and CN2-Cbi of 6, 7, 4, and 1 μM, respectively) and of cultured lymphocytes (IC50values of 60, 50, 60, and 11 μM, respectively). Experiments using cultured monocytes or lymphocytes demonstrated that OH-Cbl inhibited integration of HIV-1 DNA into cellular DNA. Thus, cobalamins and cobinamides represent novel inhibitors of HIV-1 integrase. These or related agents may be useful as anti-viral treatments that target HIV-1 integrase.

[elipoarch]

Muy interesante, Mercè, todo un hallazgo. Gracias.

[EndSFC]

Hola Merce,

Esto cuadra con lo que ya sabemos en el SFC, que hay deficiencia de las cobalaminas activas, necesarias para el ciclo de metilación y el de Krebs. La B12 es transportada a las células con glutatión y con ATP. Sabemos que el glutatión baja con el estrés continuado, y que el fallo mitocondrial evita que dispongamos de suficiente ATP. Por lo que es lógico carecer de B12...

Así pues, la conclusión sería que necesitamos restaurar el ciclo de metilación para sintetizar GSH, mejorar los niveles activos de B12, B9 y de ADP (ATP), para de esta forma poder combatir los retrovirus, al menos el VIH...No es casualidad que el glutatión se ha encontrado bajo en el Síndrome de Fatiga Crónica y en el SIDA...

Saluditos,
S.